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JoVE Journal Behavior
Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila
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Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila

Article DOI: 10.3791/56177-v • 08:30 min • October 20th, 2017
October 20th, 2017 •
1, 1,2, 1, 1
1Gene Center and Department of Biochemistry, Ludwig-Maximilians-University Munich, 2Janelia Farm Research Campus, Howard Hughes Medical Institute

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Summary
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Cells display different morphologies and establish a variety of interactions with their neighbors. This protocol describes how to reveal the morphology of single cells and to investigate cell-cell interaction by using the well-established Gal4/UAS expression system.

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MultiColor FlpOut Technique Single Cell Morphologies Cell Interactions Glia In Drosophila Visualize Anatomical Tissues Fruit Fly Developmental Stage MCFO Technique FlpOut Technique Heat Shock FLP Recombinase Reporters UAS Control Myristoylated Superfolder GFP Epitope Tag Spaghetti Monster GFPs Antibodies Excisions HSP Promoter Crosses Vials Heat Shock
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