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Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles
JoVE Journal
Biology
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JoVE Journal Biology
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles
DOI:

06:43 min

March 31, 2018

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Chapters

  • 00:00Title
  • 00:32Fixation and resin embedding
  • 02:53Trimming and sectioning of blocks
  • 03:45Staining IFM sections
  • 04:06Mounting IFM sections
  • 04:26Acquisition of images and quantification of muscle area
  • 05:10Representative results
  • 06:05Conclusions

Summary

Automatic Translation

We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.

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