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Journal / Immunology and Infection / En multi godt Format polyacrylamid-baseret…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
En multi godt Format polyacrylamid-baseret analyse for at undersøge effekten af ekstracellulære Matrix stivhed på bakteriel infektion af vedhængende celler
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En multi godt Format polyacrylamid-baseret analyse for at undersøge effekten af ekstracellulære Matrix stivhed på bakteriel infektion af vedhængende celler

Article DOI: 10.3791/57361-v • 11:51 min • July 5th, 2018
July 5th, 2018 •
1, 1, 2, 3
1Department of Biochemistry, Stanford University School of Medicine, 2Department of Mechanical and Aerospace Engineering, University of California San Diego, 3Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute, Stanford University School of Medicine

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Vi har udviklet en multi godt format polyacrylamid-baseret analyse for sondering effekten af ekstracellulære matrix stivhed på bakteriel infektion af vedhængende celler. Denne analyse er kompatibel med flowcytometri, immunfarvning og trækkraft force mikroskopi, giver mulighed for kvantitative målinger af de biomekaniske interaktion mellem celler, deres ekstracellulære matrix og sygdomsfremkaldende bakterier.

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Immunologi og infektion sag 137 tilhænger pattedyrceller bakteriel infektion endotel flowcytometri trækkraft force mikroskopi polyacrylamid hydrogels immunfarvning substrat stivhed
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