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Immunology and Infection

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JoVE Journal Immunology and Infection
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Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy

Article DOI: 10.3791/57365-v • 09:06 min • August 10th, 2018
August 10th, 2018 •
1, 1
1Department of Biological Sciences, Auburn University

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Summary
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Here we provide a method for identifying and isolating large numbers of GM-CSF driven myeloid cells using high speed cell sorting. Five distinct populations (Common myeloid progenitors, granulocyte/macrophage progenitors, monocytes, monocyte-derived macrophages, and monocyte-derived DCs) can be identified based on Ly6C and CD115 expression.

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Bone Marrow Myeloid Progenitors Dendritic Cell Precursors Cell Sorting Developmental Immunology Therapeutic Bone Marrow Transplantation Protocol Hind Legs Torso Skin Cuts Muscle Removal Femur Knee Tibia
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