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Immunology and Infection

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Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

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Immunology and Infection

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JoVE Journal Immunology and Infection

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

DOI:

10.3791/57662-v

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08:49 min

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February 14, 2019

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Sara Olivera-Ardid*¹, Nailya Khasbiullina*^2,3, Alexey Nokel, Andrey Formanovsky, Inna Popova, Tatiana Tyrtysh, Roman Kunetskiy, Nadezhda Shilova, Nicolai Bovin⁴, Daniel Bello-Gil, Rafael Mañez⁵

¹Infectious Pathology and Transplantation Division,Bellvitge Biomedical Research Institute (IDIBELL), ²Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBCh),Russian Academy of Sciences, ³Semiotik LLC, ⁴Auckland University of Technology, ⁵Intensive Care Department,Bellvitge University Hospital

Chapters

00:04Title
00:41Glycochips Production
01:39Glycan Array Technique
04:44Analysis of Glycan Array
06:09Results: Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in BALB/c Mice
07:26Conclusion

Summary

Automatic Translation

This work shows the potential of printed glycan array (PGA) technology for the analysis of circulating anti-carbohydrate antibodies in small animals.

Tags

Keywords: Glycan Array Anti-carbohydrate Antibodies Small Animals Serum Printed Glycan Array Glycan Targets Polysaccharides PBS N-hydroxy 6 Cinnamic Derivatized Glass Slides Blocking Buffer Buffer Mice Serum Immunoglobulin Aggregation Centrifugation Incubation Chamber

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