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JoVE Journal
Immunology and Infection

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巨噬细胞内细胞内生长的定量化是评价利什曼原虫寄生虫毒力的快速可靠方法
 
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巨噬细胞内细胞内生长的定量化是评价利什曼原虫寄生虫毒力的快速可靠方法

Article DOI: 10.3791/57486-v 10:01 min March 16th, 2018
March 16th, 2018

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Summary

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所有致病性的利什曼原虫物种在其脊椎动物宿主的巨噬细胞内栖息和复制。在这里, 我们提出了一个协议, 以感染小鼠骨髓源性巨噬细胞的文化与利什曼原虫, 然后精确定量的细胞内生长动力学。该方法对于研究影响寄主病原体相互作用和利什曼原虫毒力的个体因素非常有用。

Tags

免疫学和感染 问题 133,利什曼原虫 毒力 巨噬细胞 细胞内 复制 parasitophorous 液泡
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