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Journal
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Immunology and Infection
/
利用
利什曼原虫
破译毛孔形成毒素的分子机制和功能
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using
Leishmania major
Please note that all translations are automatically generated.
Click here for the English version.
利用
利什曼原虫
破译毛孔形成毒素的分子机制和功能
DOI:
10.3791/64341-v
•
08:17 min
•
October 28, 2022
•
Chaitanya S. Haram
,
Samrat Moitra
,
Rilee Keane
,
Elana Breslav
,
Kai Zhang
,
Peter A. Keyel
1
Department of Biological Sciences
,
Texas Tech University
Chapters
00:05
Introduction
01:12
Cytotoxicity Assay
06:24
Results: Tyrode s Buffer Sensitivity to
L. major
Proteins Compared to M199 and MEK Pathway Activation in
L. major
07:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是使用
利什曼原虫主要
前鞭毛体来确定孔形成毒素诱导的结合、细胞毒性和信号传导的方案。提供了链球菌溶血素O的概念验证。其他毒素也可用于利用
杆菌
中可用的遗传突变体来确定毒素抗性的新机制。
Tags
Leishmania Major
Pore-forming Toxins
Cytotoxicity Assay
Membrane-perturbing Agents
Amphotericin B
Propidium Iodide
Flow Cytometry
Serial Dilution
Promastigotes
Membrane Damage
Cell Viability
Article
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