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JoVE Journal Cancer Research
Modellazione di Osteosarcoma con la sindrome di Li-Fraumeni paziente-derivato indotto Pluripotent Stem Cells
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Modellazione di Osteosarcoma con la sindrome di Li-Fraumeni paziente-derivato indotto Pluripotent Stem Cells

Article DOI: 10.3791/57664-v • 08:52 min • June 13th, 2018
June 13th, 2018 •
1,2, 1, 1,3, 1, 4, 1, 1,2,5,6
1Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, 2Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, 3Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, 4Women's Health Institute, Cleveland Clinic Foundation, 5Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, 6Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

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Qui, presentiamo un protocollo per la generazione di induced pluripotent Stem Cells (iPSCs) da fibroblasti derivata pazienti di sindrome di Li-Fraumeni (LFS), differenziazione delle iPSCs tramite cellule staminali mesenchimali (MSCs) di osteoblasti e modellazione in vivo tumorigenesi utilizzando osteoblasti derivati paziente LFS.

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Ricerca sul cancro problema 136 iPSCs sindrome di Li-Fraumeni riprogrammazione differenziazione le cellule staminali mesenchimali osteoblasti osteosarcoma dello xenotrapianto
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