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Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice
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Chapters
- 00:05Introduction
- 00:46Fabricating and Assembly of the Acoustofluidic Microdevice
- 03:02Sample Preparation
- 05:41Measuring the Compressibility of Cell and Nucleus
- 07:29Results: Measuring Cell or Nucleus Compressibility Based on Acoustofluidic Microdevice
- 08:38Conclusion
Here a protocol is presented to build a fast and non-destructive system for measuring cell or nucleus compressibility based on acoustofluidic microdevice. Changes in mechanical properties of tumor cells after epithelial-mesenchymal transition or ionizing radiation were investigated, demonstrating the application prospect of this method in scientific research and clinical practice.
Tags
Keywords:
Cell MechanicsTumor MetastasisMalignant TransformationRadiosensitivityAcoustofluidic MethodSuspended StatesEpithelial To Mesenchymal TransitionCirculating Tumor CellsCancer DiagnosisPDMSPlasma CleanerPTFE CatheterStainless Steel NeedlePiezoelectric CeramicCyanoacrylate GluePolystyrene Standard Particle SolutionsCell Suspensions
