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JoVE Journal Bioengineering
Stivhed måling af blød silikone underlag for Mechanobiology undersøgelser ved hjælp af en Widefield fluorescens mikroskop
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Stivhed måling af blød silikone underlag for Mechanobiology undersøgelser ved hjælp af en Widefield fluorescens mikroskop

Article DOI: 10.3791/57797-v • 07:02 min • July 3rd, 2018
July 3rd, 2018 •
*1, *1, *2, *1, 1, 1
1Department of Mechanical & Aerospace Engineering, Old Dominion University, 2Department of Biological Sciences, Old Dominion University
* These authors contributed equally

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Substrater med stivhed i kilopascal-området er nyttige at studere svar af celler til fysiologisk relevante mikro-miljø stivhed. Bruger bare en widefield fluorescens mikroskop, kan de unge modulus af blød silikone gel bestemmes ved hjælp af en indrykning med en egnet kugle.

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Bioteknologi sag 137 indrykning Polydimethylsiloxan trækkraft force mikroskopi fluorescens mechanobiology biomekanik
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