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Expression and Purification of Mammalian Bestrophin Ion Channels
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Biochemistry
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JoVE Journal Biochemistry
Expression and Purification of Mammalian Bestrophin Ion Channels

Expression and Purification of Mammalian Bestrophin Ion Channels

DOI:
10.3791/57832-v

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08:12 min

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August 02, 2018

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, , , ,
1Department of Pharmacology and Physiology,University of Rochester, School of Medicine and Dentistry

Chapters

  • 00:04Title
  • 00:43Protein Expression
  • 03:00Protein Purification
  • 07:02Results: Expression and Purification of a Mammalian Bestrophin Protein
  • 07:32Conclusion

Summary

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The purification of ion channels is often challenging, but once achieved, it can potentially allow in vitro investigations of the functions and structures of the channels. Here, we describe the stepwise procedures for the expression and purification of mammalian bestrophin proteins, a family of Ca2+-activated Cl- channels.

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Mammalian Bestrophin Ion ChannelsProtein ExpressionPurificationHEK293F CellsBaculovirusSodium ButyrateCell DensityCell ViabilityProtein Purification

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