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Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism

Article DOI: 10.3791/58430-v • 09:10 min • January 12th, 2019
January 12th, 2019 •
1, 1, 1, 1, 1, 1, 2, 3, 4, 5, 1,6, 1,3,6,7
1Department of Surgery, Faculty of Medicine, University of Alberta, 2Department of Pediatrics, Faculty of Medicine, University of Alberta, 3Department of Biomedical Engineering, Faculty of Medicine, University of Alberta, 4Department of Mechanical Engineering, Faculty of Engineering, University of Alberta, 5Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, 6Canadian National Transplant Research Program, 7Department of Physiology, Faculty of Medicine, University of Alberta

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Summary
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Normothermic ex situ heart perfusion (ESHP), preserves the heart in a beating, semi-physiologic state. When performed in a working mode, ESHP provides the opportunity to perform sophisticated assessments of donor heart function and organ viability. Here, we describe our method for myocardial performance evaluation during ESHP.

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Normothermic Ex Situ Heart Perfusion Working Mode Cardiac Function Metabolic Evaluation Transplantation Suitability Functional Evaluation Donor Hearts Therapy Of Dysfunctional Donor Hearts Technical Expertise Visual Demonstration Heart Retrieval Connection To The Device Median Sternotomy Anesthetized Pig Pericardium Silk Sutures Sternum Whole Blood Collection Venous Cannula Isotonic Crystalloid Solution Perfusion Circuit
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