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JoVE Journal Neuroscience
Vorbereitung der akuten Rückenmark Scheiben auf Whole-Cell Patch-Clamp-Aufnahme in den Neuronen der Substantia Gelatinosa
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Vorbereitung der akuten Rückenmark Scheiben auf Whole-Cell Patch-Clamp-Aufnahme in den Neuronen der Substantia Gelatinosa

Article DOI: 10.3791/58479-v • 08:30 min • January 18th, 2019
January 18th, 2019 •
1, 1, 2, 2, 2, 2, 2,3
1Department of Pain, First Affiliated Hospital of Nanchang University, 2Department of Pediatrics, First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

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Hier beschreiben wir die wesentlichen Schritte für die ganze Zelle Patch-Clamp-Aufnahmen, die von Neuronen der Substantia Gelatinosa (SG) in der in-vitro- Rückenmark-Slice. Diese Methode ermöglicht die innere Membran Eigenschaften, synaptische Übertragung und morphologische Charakterisierung der SG Neuronen untersucht werden.

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Neurowissenschaften Ausgabe 143 Neurowissenschaften Rückenmark Slice Substantia Gelatinosa Neurons ganze Zelle Patch-Clamp Elektrophysiologie Morphologie in vitro
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