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Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

DOI:
10.3791/58592-v

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11:21 min

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November 20, 2018

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, ,
1Department of Pharmacology,University College London

Chapters

  • 00:04Title
  • 00:26Tissue Preparation and Staining for Fluorescence Microscopy and 3D Neuronal Reconstruction
  • 04:08Histological Detection of Biotin-filled Neurons
  • 08:033D Neuronal Reconstructions
  • 10:12Results: Reconstruction of a Basket Cell Recorded and Filled in the Hippocampal CA2 Region and Morphometry Analyses
  • 10:57Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol outlined here describes the immunofluorescence analysis, biocytin recovery and high-quality reconstructions of hippocampal CA2 interneurons following the intracellular electrophysiological recordings in vitro, allowing neuronal characterization and ultimately fine neuronal anatomy to be studied.

Tags

Keywords: Biocytin3D ReconstructionHippocampal CA2 InterneuronsNeuronal SubtypesCortical CircuitryUltrastructureDendritic ArborAxonal ArborElectrophysiologyACSFFixative SolutionPhosphate BufferGelatinVibratomeSucrose

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