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JoVE Journal
Neuroscience

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Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
 

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Article doi: 10.3791/58592
November 20th, 2018

Summary November 20th, 2018

The protocol outlined here describes the immunofluorescence analysis, biocytin recovery and high-quality reconstructions of hippocampal CA2 interneurons following the intracellular electrophysiological recordings in vitro, allowing neuronal characterization and ultimately fine neuronal anatomy to be studied.

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