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Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
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Chapters
- 00:04Title
- 00:31Cell Seeding, Virus Infection, and Immunofluorescent Labeling
- 03:12Confocal Imaging and Nuclear vs. Cytoplasmic Distribution Analysis
- 05:12Results: Representative Infected Cell Protein 0 (ICP0) Distribution Analyses
- 05:56Conclusion
ICP0 undergoes nuclear-to-cytoplasmic translocation during HSV-1 infection. The molecular mechanism of this event is not known. Here we describe the use of confocal microscope as a tool to quantify ICP0 movement in HSV-1 infection, which lays the groundwork for quantitatively analyzing ICP0 translocation in future mechanistic studies.
