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Using High Content Imaging to Quantify Target Engagement in Adherent Cells
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Biochemistry
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JoVE Journal Biochemistry
Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

DOI:
10.3791/58670-v

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07:23 min

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November 29, 2018

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, ,
1Chemical Biology Consortium Sweden, Science for Life Laboratory,Karolinska Institutet, Solna, 2Department of Medical Biochemistry and Biophysics,Karolinska Institutet, Solna, 3Science for Life Laboratory, Department of Oncology-Pathology,Karolinska Institutet, Stockholm

Chapters

  • 00:04Title
  • 00:31Cell Seeding
  • 01:51Compound Treatment and Heat Challenge
  • 03:30Staining, Image Acquisition and Analysis
  • 05:33Results: Representative Target Engagement Quantification by High Content Imaging
  • 06:54Conclusion

Summary

Automatic Translation

Automatic Translation

Measurements of drug target engagement are central to effective drug development and chemical probe validation. Here, we detail a protocol for measuring drug-target engagement using high content imaging in a microplate-compatible adaption of the cellular thermal shift assay (CETSA).

Tags

High Content ImagingTarget EngagementAdherent CellsChemical BiologyDrug DiscoveryCell LinesPrimary CulturesCoculturesA-431 CellsTrypsinPlate WashingCompound TreatmentHeat ChallengeWater Bath

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