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Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
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Cancer Research
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JoVE Journal Cancer Research
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

DOI:
10.3791/58758-v

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09:07 min

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December 19, 2018

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,
1Reproductive Developmental Biology Laboratory, National Institute of Environmental Health Sciences,National Institutes of Health (NIH)

Chapters

  • 00:04Title
  • 00:42Mammalian Two-Hybrid Assay
  • 02:19DNA Mixture Preparation
  • 03:49Transfection and Dual-Luciferase Assay
  • 06:04Results: Representative Ligand-Dependent Dimerization of Mouse Estrogen Receptor Alpha-Ligand Binding Domain (ERalpha-LBD)
  • 08:41Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method for analyzing the 4-hydroxy-tamoxifen-dependent estrogen receptor alpha ligand-binding domain dimerization activity using the mammalian two-hybrid assay.

Tags

Estrogen Receptor AlphaLigand-binding DomainDimerizationMammalian Two-hybrid AssayLuciferase ReporterPBINDPACTSelective Estrogen Receptor Modulators

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