Journal
/
/
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
DOI:

09:07 min

December 19, 2018

,

Chapters

  • 00:04Title
  • 00:42Mammalian Two-Hybrid Assay
  • 02:19DNA Mixture Preparation
  • 03:49Transfection and Dual-Luciferase Assay
  • 06:04Results: Representative Ligand-Dependent Dimerization of Mouse Estrogen Receptor Alpha-Ligand Binding Domain (ERalpha-LBD)
  • 08:41Conclusion

Summary

Automatic Translation

We present a method for analyzing the 4-hydroxy-tamoxifen-dependent estrogen receptor alpha ligand-binding domain dimerization activity using the mammalian two-hybrid assay.

Related Videos

Read Article