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Immunology and Infection
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Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
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Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster

Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster

DOI:
10.3791/58845-v

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09:57 min

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March 14, 2019

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, , , , , ,
1The Joint Center for Infection and Immunity, Guangzhou Institute of Pediatrics,Guangzhou Women and Children’s Medical Center, 2CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai,Chinese Academy of Sciences, 3CAS Key Laboratory of Infection and Immunity (CASKLII), Institute of Biophysics,Chinese Academy of Sciences, 4Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences

Chapters

  • 00:04Title
  • 00:31Virus Propagation and Collection
  • 02:10Infective Dose Determination
  • 03:48Wolbachia-Free Fly Preparation and Infection
  • 08:00Results: Representative Host-Virus Interactions in DCV-Infected Drosophila
  • 08:47Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to establish viral infection in vivo in Drosophila melanogaster using the nano-injection method and basic techniques to analyze virus-host interaction.

Tags

Viral InfectionDrosophila MelanogasterHost-virus InteractionNanoinjectionVirus-host InteractionS2 Star CellsDrosophila C VirusDCVCell MorphologyCell DebrisVirus ConcentrationVirus Dilution

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