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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
DOI:

10:58 min

March 05, 2019

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Chapters

  • 00:04Title
  • 00:32Cell Preparation
  • 02:15Cell Staining – General Procedure
  • 04:02Antibody Titration
  • 06:41Gating Strategy
  • 08:15Results: Analysis of Samples from Patients with Multiple Myeloma
  • 10:02Conclusion

Summary

Automatic Translation

Here, we present a flow cytometric protocol to identify CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes in human peripheral blood by using only two fluorochromes instead of seven. With this approach, five additional markers can be recorded on most flow cytometers.

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