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An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
JoVE Journal
Biology
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JoVE Journal Biology
An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
DOI:

09:47 min

February 01, 2019

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Chapters

  • 00:04Title
  • 01:05Generation of LN215-YFPQL and LN215-I” Cells by Lentiviral Transduction
  • 03:14Plating the LN215-YFPQL and LN215-I Cells
  • 04:42Conducting the I-YFP GJIC Assay
  • 07:37Results: Analysis of the Iodine-Yellow Fluorescent Protein-Gap Junction Intercellular Communication Assay
  • 08:15Conclusion

Summary

Automatic Translation

Here, we present a protocol for a novel gap junction intercellular communication assay designed for the high-throughput screening of gap junction-modulating chemicals for drug discovery and toxicological assessment.

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