Journal
/
Immunology and Infection
/
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

DOI:
10.3791/59124-v

•

09:29 min

•

March 05, 2019

•

, , , ,
1Division of Clinical Pharmacology, Department of Medicine,Vanderbilt University Medical Center

Chapters

  • 00:04Title
  • 00:52Isolation of Spleens from Mice
  • 01:27Generation of Single Cell Suspensions from Spleens
  • 02:39Isolation of CD11c+ DCs from Splenic Single Cell Suspension
  • 03:41Magnetic Separation of CD11c+ DCs
  • 04:51Adoptive Transfer of High Salt Treated CD11c+ DCs
  • 06:56Results: Analysis of the High Salt Treated Antigen-presenting Dendritic Cells
  • 08:52Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to isolate dendritic cells from murine spleens by magnetic cell sorting and subsequent adoptive transfer into naïve mice. The model of high-salt activated dendritic cells was chosen to explain the step-by-step procedures of adoptive transfer and flow cytometry.

Tags

Dendritic CellsCD11cHypertensionCardiovascular DiseaseAdoptive TransferSpleenIsolationCollagenase DDNaseMACSFlow Cytometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

Isolation of Mouse Lung Dendritic Cells

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells

Read Article