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Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
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Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
DOI:

05:56 min

May 15, 2019

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Chapters

  • 00:04Title
  • 01:02Potato Plant and Insect Preparation
  • 02:02Infestation Experiment Setup
  • 03:14Infestation and Leaf Harvest
  • 04:16Results: Representative Leaf Damage and Gene Expression
  • 05:23Conclusion

Summary

Automatic Translation

The presented method creates natural herbivore damaged plant tissue through the application of Manduca sexta larvae to detached leaves of potato. The plant tissue is assayed for expression of six transcription factor homologs involved in early responses to insect herbivory.

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