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Swimming Induced Paralysis to Assess Dopamine Signaling in Caenorhabditis elegans
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JoVE Journal Behavior
Swimming Induced Paralysis to Assess Dopamine Signaling in Caenorhabditis elegans

Swimming Induced Paralysis to Assess Dopamine Signaling in Caenorhabditis elegans

DOI:
10.3791/59243-v

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07:36 min

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April 03, 2019

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, ,
1Harriet Wilkes Honors College,Florida Atlantic University, John D MacArthur Campus, 2Integrative Biology and Neuroscience program, College of Science,Florida Atlantic University, 3Brain Institute,Florida Atlantic University, 4Department of Biomedical Science, Charles E. Schmidt College of Medicine,Florida Atlantic University

Chapters

  • 00:04Title
  • 00:48C. elegans Husbandry
  • 04:12Swimming-induced Paralysis (SWIP)
  • 06:13Results: Representative Manual and Automated Amphetamine-induced SWIP Analysis
  • 06:57Conclusion

Summary

Automatic Translation

Automatic Translation

Swimming induced paralysis (SWIP) is a well-established behavioral assay used to study the underlying mechanisms of dopamine signaling in Caenorhabditis elegans (C. elegans). However, a detailed method to perform the assay is lacking. Here, we describe a step-by-step protocol for SWIP.

Tags

Swimming Induced ParalysisSWIPDopamine SignalingCaenorhabditis ElegansBehavioral AssayDopaminergic SynapsisDopamine TransporterSynchronized PopulationSodium Hypochloride Sodium Hydroxide SolutionEgg BufferDensity Gradient Separation

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