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Journal / Developmental Biology / Realtids Live-celle flowcytometri at undersøge…
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Developmental Biology

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JoVE Journal Developmental Biology
Realtids Live-celle flowcytometri at undersøge Calcium tilstrømning, Pore dannelse og fagocytose af P2X7 receptorer i voksne neurale stamceller
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Realtids Live-celle flowcytometri at undersøge Calcium tilstrømning, Pore dannelse og fagocytose af P2X7 receptorer i voksne neurale stamceller

Article DOI: 10.3791/59313-v • 11:47 min • April 3rd, 2019
April 3rd, 2019 •
1,2, 3,4, 3,5,6, 7, *1,4,7, *8
1Griffith Institute for Drug Discovery, Griffith University, 2Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science, University of Sydney, 4Bosch Institute, University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit, St. Vincent's Centre for Applied Medical Research, 6School of Medical Sciences, The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science, Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health, University of Melbourne
* These authors contributed equally

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Giver én celle følsomhed, er real-time flowcytometri unikt tilpasset til at kvantificere multimodale receptor funktioner af levende kulturer. Ved hjælp af voksne neurale stamceller, blev P2X7 receptor funktionen vurderet via calcium tilstrømning registreres af calcium indikator farvestof, transmembrane pore dannelsen af ethidium bromid optagelse og fagocytose ved hjælp af fluorescerende latex perler.

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Udviklingsmæssige biologi spørgsmålet 146 P2X7 receptorer flowcytometri calcium purinergic signalering fagocytose pore dannelse neurale stamceller
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