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Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
DOI:

09:20 min

May 14, 2019

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Chapters

  • 00:04Title
  • 01:06Embryo Embedding and Preparation of Cryosections
  • 02:31Immunofluorescence for pH3
  • 04:54Immunohistochemistry for Labeled Dextran
  • 07:50Results: Identification of Individual Cells After Immunofluorescence and Immunohistochemistry
  • 08:21Conclusion

Summary

Automatic Translation

This protocol demonstrates sequential immunofluorescence and immunohistochemistry on cryosections from early-stage zebrafish embryos enabling precise colocalization analyses in specific cell populations.

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