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Journal / Biochemistry / 使用基于化学探针的免疫测定直接测量KDM1A目标参与…
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Biochemistry

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JoVE Journal Biochemistry
使用基于化学探针的免疫测定直接测量KDM1A目标参与
Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
Journal (Biochemistry)
Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
Journal (Biochemistry)
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
 
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使用基于化学探针的免疫测定直接测量KDM1A目标参与

Article DOI: 10.3791/59390-v • 11:17 min • June 13th, 2019
June 13th, 2019 •
1, 1, 1
1Oryzon Genomics

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在这里,我们提出一个协议,以测量KDM1A靶向在使用KDM1A抑制剂处理的人或动物细胞、组织或血液样本中的参与度。该协议采用自由KDM1A酶的化学探针标记,并使用基于化学探针的免疫测定直接定量目标职业,可用于临床前和临床研究。

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生物化学 问题 148 目标参与 组蛋白修饰 组蛋白脱甲基酶 KDM1A 化学探针 ELISA
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