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使用 HPLC-MS/MS 进行核苷/核苷酸含量测量的工厂样品制备
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Biochemistry
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JoVE Journal Biochemistry
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
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使用 HPLC-MS/MS 进行核苷/核苷酸含量测量的工厂样品制备

DOI:
10.3791/61956-v

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06:38 min

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February 24, 2021

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, , , , , , , ,
1College of Life Sciences,Nanjing Agricultural University, 2Department of Criminal Science and Technology,Nanjing Forest Police College, 3Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition,Leibniz University Hannover

Chapters

  • 00:04Introduction
  • 00:54Nucleosides/Nucleotides Extraction
  • 02:57LC-MS/MS Measurement
  • 04:27Results: Identification of N1-Methyladenosine by Mass Transition
  • 05:57Conclusion

Summary

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此处描述了植物体内核苷酸/核苷酸定量的精确和可重复的方法。此方法采用 HPLC-MS/MS。

Tags

Plant Sample PreparationNucleosidesNucleotidesHPLC-MS/MSMetabolite ExtractionArabidopsisLC-MS/MSAmmonium Acetate BufferMethanolAcetonitrile

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