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Journal / Developmental Biology / iPSC Kaynaklı Kas Atalarında Distrofin…
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JoVE Journal Developmental Biology
iPSC Kaynaklı Kas Atalarında Distrofin Ekspresyonunun Geri Kazanilmesinde CRISPR/Cas9 Teknolojisi
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iPSC Kaynaklı Kas Atalarında Distrofin Ekspresyonunun Geri Kazanilmesinde CRISPR/Cas9 Teknolojisi

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

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Burada, Dmdmdx fare kaynaklı deri fibroblastlarından iPSC'de distrofin ekspresyonunu geri yüklemek ve Tet-on MyoD aktivasyon sistemini kullanarak iPSC'leri doğrudan miyojenik progenitor hücrelerine (MPC) ayırmak için Cas9 tabanlı ekson23 silme protokolü salıyoruz.

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Gelişimbiyolojisi Sayı 151 indüklenen pluripotent kök hücreler iPSC CRISPR/Cas9 DMD distrofin miyojenik ata diferansiyasyon
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