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JoVE Journal Developmental Biology
CRISPR/Cas9 technologie in het herstellen van Dystrofine expressie in door iPSC afgeleide spier voorlopercellen
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CRISPR/Cas9 technologie in het herstellen van Dystrofine expressie in door iPSC afgeleide spier voorlopercellen

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

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Hier presenteren we een Cas9-gebaseerd exon23 deletie protocol om Dystrofine expressie in iPSC te herstellen van DMDMDX Mouse-afgeleide huid fibroblasten en direct ipscs te onderscheiden in myogene voorlopercellen (MPC) met behulp van het Tet-on myod activeringssysteem.

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Ontwikkelingsbiologie uitgave 151 geïnduceerde pluripotente stamcellen iPSC CRISPR/Cas9 DMD Dystrofine myogene voorloper differentiatie
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