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JoVE Journal Developmental Biology
CRISPR/Cas9-teknologi til genoprettelse af dystrophin-ekspression i iPSC-afledte muskel Progenitorer
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CRISPR/Cas9-teknologi til genoprettelse af dystrophin-ekspression i iPSC-afledte muskel Progenitorer

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

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Her præsenterer vi en Cas9-baseret exon23 sletnings protokol for at genoprette dystrofin Expression i IPSC fra DMDMDX Mouse-afledte hudfibroblaster og direkte differentiere ipscs i myogenic stamceller (MPC) ved hjælp af tet-on myod aktiveringssystem.

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Udviklingsbiologi inducerede pluripotente stamceller iPSC CRISPR/Cas9 DMD dystrofin myogen Progenitor differentiering
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