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Journal / Developmental Biology / CRISPR/Cas9在iPSC衍生肌肉预祖中恢复肌营养不良素表达的技术…
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Developmental Biology

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JoVE Journal Developmental Biology
CRISPR/Cas9在iPSC衍生肌肉预祖中恢复肌营养不良素表达的技术
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CRISPR/Cas9在iPSC衍生肌肉预祖中恢复肌营养不良素表达的技术

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

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在这里，我们提出了基于Cas9的外显23删除协议，从Dmdmdx小鼠衍生的皮肤成纤维细胞恢复iPSC中的营养不良素表达，并使用Tet-on MyoD激活系统将iPSC直接分化为肌源性祖细胞（MPC）。

Tags

发育生物学，第151期，诱导多能干细胞，iPSC，CRISPR/Cas9，DMD，营养不良素，肌源性祖细胞，分化
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