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Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor
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Bioengineering
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JoVE Journal Bioengineering
Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor

Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor

DOI:
10.3791/59476-v

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07:20 min

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April 25, 2019

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, ,
1Department of Physics and Astronomy,Iowa State University, 2Department of Physics and Astronomy, Molecular, Cellular, and Developmental Biology Interdepartmental Program,Iowa State University

Chapters

  • 00:04Title
  • 00:36Integrative Tension Sensor Synthesis
  • 02:58Integrative Tensor Sensor (ITS) Immobilization
  • 03:58Cell Plating
  • 05:14Quasi-real Time Live Cell Integrin Tension Imaging
  • 06:05Results: Representative ITS Imaging
  • 06:52Conclusion

Summary

Automatic Translation

Automatic Translation

Integrin tension plays important roles in various cell functions. With an integrative tension sensor, integrin tension is calibrated with picoNewton (pN) sensitivity and imaged at submicron resolution.

Tags

Integrin Tension SensorDNA-based Integrative Tension SensorFluorescence MicroscopyCellular ForceRGD PeptideTCEPSulfo-SMCCDNA ConjugationITS SynthesisBSA-biotinGlass-bottom Petri Dish

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