JoVE Journal
Biochemistry
Biochemistry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Cultured primary or established cell lines are commonly used to address fundamental biological and mechanistic questions as an initial approach before using animal models. This protocol describes how to prepare whole cell extracts and subcellular fractions for studies of zinc (Zn) and other trace elements with atomic absorbance spectroscopy.