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Journal / Genetics / BK-多瘤病毒非编码控制区域驱动转录活性通过流细胞测定的测量…
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Genetics
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JoVE Journal Genetics
BK-多瘤病毒非编码控制区域驱动转录活性通过流细胞测定的测量
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BK-多瘤病毒非编码控制区域驱动转录活性通过流细胞测定的测量

Article DOI: 10.3791/59755-v • 11:54 min • July 13th, 2019
July 13th, 2019 •
1,2, 2, 2, 2,3, 3, 3, 4, 1, 2, 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

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在本手稿中,提出了使用 HEK293T 细胞转染的基于 FACS 的 BK-多瘤病毒转录活性的测量方案,该细胞与表达 tdTomato 和 eGFP 的双向报告质粒转染。该方法进一步允许定量确定新化合物对病毒转录的影响。

Tags

遗传学 问题 149 BK-Polyoma病毒 BKPyV 非编码控制区 NCCR 双向促进剂活性 FACS 抗病毒活性 肾移植 大T抗原 SV40
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