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Journal / Immunology and Infection / Lamina Propria Mononükleer Hücrelerin Murine…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Lamina Propria Mononükleer Hücrelerin Murine Kolondan Kollajenaz E Kullanarak İzolasyon
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Lamina Propria Mononükleer Hücrelerin Murine Kolondan Kollajenaz E Kullanarak İzolasyon

Article DOI: 10.3791/59821-v • 09:48 min • September 26th, 2019
September 26th, 2019 •
1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4,5
1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation, University of Miami Miller School of Medicine, 2Batchelor Children's Research Institute, University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology, University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 5Holtz Children's Hospital, University of Miami Miller School of Medicine

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Bu protokolün amacı kollajenaz kullanarak dokunun enzimatik sindirimi ile kolon lamina propria bulunan mononükleer hücreleri izole etmektir. Bu protokol, mononükleer hücrelerin verimli bir şekilde izolasyonuna olanak sağlar ve bu da sağlam immünhenototipleme için kullanılabilir tek bir hücre süspansiyonu ile sonuçlanır.

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İmmünoloji ve Enfeksiyon Sayı 151 Lenfositler kolon murine lamina propria kolit transplantasyon akış sitometrisi enzimatik sindirim inflamasyon mononükleer hücreler
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