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A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

DOI:
10.3791/64634-v

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05:44 min

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December 09, 2022

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1The Rausing Laboratory, Autoimmunity Section, Division of Neurosurgery, Department of Clinical Sciences,Lund University, 2Department of Autoimmunity,BTB Emulsions

Chapters

  • 00:04Introduction
  • 00:59Material Preparation
  • 01:38Preparation of CFA/Peptide Emulsions
  • 03:31Quality Control of Emulsions
  • 04:01Results: Characterization of Emulsions Produced and EAE Induction in Immunized Mice
  • 05:02Conclusion

Summary

Automatic Translation

Automatic Translation

To induce experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis, mice are immunized with a water-in-oil emulsion containing an autoantigen and complete Freund's adjuvant. While several protocols exist for the preparation of these emulsions, a rapid, simple, and standardized homogenization protocol for emulsion preparation is presented here.

Tags

Keywords: Homogenization MethodAntigen/adjuvant EmulsionExperimental Autoimmune EncephalomyelitisEAECFA SlurryIFA AdjuvantEmulsion PreparationAutoimmune Disease ModelCancer Vaccines

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