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Journal / Biochemistry / Mätning av interaktioner mellan fluorescerande…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Mätning av interaktioner mellan fluorescerande sonder och lignin i Plant sektioner av sFLIM baserat på ursprunglig Autofluorescence
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Journal (Biochemistry)
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Journal (Biochemistry)
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
 
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Mätning av interaktioner mellan fluorescerande sonder och lignin i Plant sektioner av sFLIM baserat på ursprunglig Autofluorescence

Article DOI: 10.3791/59925-v • 07:15 min • January 2nd, 2020
January 2nd, 2020 •
1, 2, 2, 3
1PICT Platform, Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille

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Detta protokoll beskriver en ursprunglig inställning som kombinerar spektral-och fluorescenslivstids mätningar för att utvärdera Förster resonans energiöverföring (FRET) mellan rhodamine-baserade fluorescerande sonder och lignin polymer direkt i tjocka Plant sektioner.

Tags

Biokemi sFLIM fluorescens livstid FRET Autofluorescence lignocellulosa interaktion
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