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Journal
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Biochemistry
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基于原生自荧光的sFLIM测量植物部分荧光探针和木质素之间的相互作用
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
Please note that all translations are automatically generated.
Click here for the English version.
基于原生自荧光的sFLIM测量植物部分荧光探针和木质素之间的相互作用
DOI:
10.3791/59925-v
•
00:07 min
•
January 02, 2020
•
Christine Terryn
,
Anouck Habrant
,
Gabriel Paës
,
Corentin Spriet
1
PICT Platform
,
Université de Reims Champagne-Ardenne
,
2
Fractionation of AgroResources and Environment (FARE) Laboratory, INRA
,
Université de Reims Champagne-Ardenne
,
3
Unité de Glycobiologie Structurale et Fonctionnelle
,
Université de Lille
Chapters
00:04
Title
00:54
Plant Sample Preparation
02:21
Spectrally-resolved Fluorescence Lifetime and Spectral Measurement (sFLIM) System Calibration
03:09
sFLIM Measurements
04:13
sFLIM Analysis
05:26
Results: Representative sFLIM Measurements
06:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了结合光谱和荧光寿命测量的原始设置,以评估在厚植物部分直接测定基于红胺的荧光探针和木质素聚合物之间的Fürster共振能量转移(FRET)。
Tags
Fluorescent Probes
Lignin
Plant Sections
SFLIM
Native Autofluorescence
FRET Analysis
Lignocellulosic Biomass
Enzyme Activity
Polysaccharides
Sample Sectioning
Multidimensional Microscopy
PEG Embedding
Microtome
Fluorescent Staining
Spectral Window
SFLIM Detector
Titanium-doped Sapphire Laser
Article
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