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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study

DOI:
10.3791/59933-v

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07:53 min

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August 16, 2019

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, , ,
1Institute for Molecular Biotechnology,RWTH Aachen University, 2Sanofi Deutschland GmbH, 3Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.

Chapters

  • 00:04Title
  • 01:05Coupling DFE to the Activated Cross-linked Agarose Resin
  • 03:43Testing the Purification of mAbs from Clarified Plants Extracts
  • 05:34Results: Affinity Chromatography Resins Developed by Activated Cross-linked Agarose
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

In this procedure, a DsRed-based epitope ligand is immobilized to produce a highly selective affinity resin for the capture of monoclonal antibodies from crude plant extracts or cell culture supernatants, as an alternative to Protein A.

Tags

Activated Cross-linked AgaroseAffinity Chromatography ResinsAntibody CaptureMonoclonal AntibodiesProtein A ChromatographyAffinity LigandHIV Neutralizing Antibody 2F5Dynamic Binding CapacityResin ReusabilityPurification CostsNHS-Activated Cross-linked AgaroseCoupling Reaction

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