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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study
DOI:

07:53 min

August 16, 2019

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Chapters

  • 00:04Title
  • 01:05Coupling DFE to the Activated Cross-linked Agarose Resin
  • 03:43Testing the Purification of mAbs from Clarified Plants Extracts
  • 05:34Results: Affinity Chromatography Resins Developed by Activated Cross-linked Agarose
  • 07:21Conclusion

Summary

Automatic Translation

In this procedure, a DsRed-based epitope ligand is immobilized to produce a highly selective affinity resin for the capture of monoclonal antibodies from crude plant extracts or cell culture supernatants, as an alternative to Protein A.

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