Journal
/
Genetics
/
Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs

Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs

DOI:
10.3791/60056-v

•

08:49 min

•

September 16, 2019

•

, ,
1Institute for Integrative Biology of the Cell, UMR9198, CNRS CEA Univ Paris-Sud,Université Paris-Saclay

Chapters

  • 00:04Title
  • 00:33Small RNA Isolation
  • 02:26Unligated 3’ Adapter Elimination
  • 04:41Gel Purification
  • 05:27Raw Sequence File Treatment
  • 06:06Trimmed Sequence Mapping
  • 06:58Results: Representative 2’-OMe RNA Detection
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

We present a detailed small RNA library reparation protocol with less bias than standard methods and an increased sensitivity for 2'-O-methyl RNAs. This protocol can be followed using homemade reagents to save cost or using kits for convenience.

Tags

Small RNA-seqRNA Detection2′-O-Methyl RNAsUnbiased MethodsPlant MicroRNAsGel ElectrophoresisRNA ExtractionAdapter EliminationMagnetic Purification Beads

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Detection of Copy Number Alterations Using Single Cell Sequencing

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Read Article