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Genetics

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Methodology for Accurate Detection of Mitochondrial DNA Methylation
 

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Article DOI: 10.3791/57772
May 20th, 2018

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Summary May 20th, 2018

Here, we present a protocol to allow accurate quantification of mitochondrial DNA (mtDNA) methylation. In this protocol, we describe an enzymatic digestion of DNA with BamHI coupled with a bioinformatic analysis pipeline which can be used to avoid overestimation of mtDNA methylation levels caused by the secondary structure of mtDNA.

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