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Journal / Biochemistry / SUMO-bindende enheder (SUBEs) som værktøjer til…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
SUMO-bindende enheder (SUBEs) som værktøjer til berigelse, isolation, identifikation og karakterisering af SUMO Proteome i leverkræft
Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
Journal (Biochemistry)
Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
Western Blotting of Digested DNA Samples Containing DNA-Protein Crosslinks (DPCs) (Video) | JoVE
Journal (Biochemistry)
Western Blotting of Digested DNA Samples Containing DNA-Protein Crosslinks (DPCs) (Video) | JoVE
 
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SUMO-bindende enheder (SUBEs) som værktøjer til berigelse, isolation, identifikation og karakterisering af SUMO Proteome i leverkræft

Article DOI: 10.3791/60098-v • 08:29 min • November 1st, 2019
November 1st, 2019 •
1, 1, 1, 2, 2, 3, 1
1Liver Disease and Liver Metabolism Lab, CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 2Proteomics Platforms, CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, 3Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS, UbiCARE

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Her præsenterer vi en protokol til at berige, isolere, identificere og karakterisere proteiner modificeret af Sumo in vivo både fra humane hepatoma celler og levertumorer opnået fra musemodeller af hepatocellulært karcinom ved hjælp af Sumo-bindende enheder (subes).

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Biokemi SUMO SUBEs leverkræft Hepatocellulær carcinoma hepatoma massespektrometri
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