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Biochemistry

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Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
 

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Article doi: 10.3791/60734
March 25th, 2020

Summary March 25th, 2020

Two methods of cholesterol enrichment are presented: the application of cyclodextrin saturated with cholesterol to enrich mammalian tissues and cells, and the use of cholesterol-enriched phospholipid-based dispersions (liposomes) to enrich Xenopus oocytes. These methods are instrumental for determining the impact of elevated cholesterol levels in molecular, cellular, and organ function.

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