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Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
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Biochemistry
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JoVE Journal Biochemistry
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

DOI:
10.3791/60734-v

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10:12 min

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March 25, 2020

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, , , , ,
1Department of Pharmacology, Addiction Science and Toxicology,The University of Tennessee HSC, 2Department of Chemistry,University of Illinois at Chicago

Chapters

  • 00:04Introduction
  • 01:02Methyl-Beta-Cyclodextrin (MBCD) Cholesterol-Enriching Solution Preparation
  • 02:12Cholesterol-Saturated Tissue Enrichment
  • 05:07Cholesterol Liposome-Enriched Phospholipid-Based Dispersion Preparation
  • 06:06Xenopus Oocyte Cholesterol Enrichment
  • 07:37Results: Representative Effects of Rat Cerebral Artery and Xenopus Oocyte Cholesterol Enrichment
  • 09:22Conclusion

Summary

Automatic Translation

Automatic Translation

Two methods of cholesterol enrichment are presented: the application of cyclodextrin saturated with cholesterol to enrich mammalian tissues and cells, and the use of cholesterol-enriched phospholipid-based dispersions (liposomes) to enrich Xenopus oocytes. These methods are instrumental for determining the impact of elevated cholesterol levels in molecular, cellular, and organ function.

Tags

Keywords: Cholesterol EnrichmentXenopus OocytesHypercholesterolemiaCellular MembranesCerebral ArteriesCircle Of WillisMethyl-beta-cyclodextrinCholesterol Saturated SolutionLipid HandlingFilipin Staining

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