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Journal / Biochemistry / SUMO-bindende entiteiten (SUBEs) als hulpmiddelen …
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
SUMO-bindende entiteiten (SUBEs) als hulpmiddelen voor de verrijking, isolatie, identificatie en karakterisering van de SUMO proteome in leverkanker
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SUMO-bindende entiteiten (SUBEs) als hulpmiddelen voor de verrijking, isolatie, identificatie en karakterisering van de SUMO proteome in leverkanker

Article DOI: 10.3791/60098-v • 08:29 min • November 1st, 2019
November 1st, 2019 •
1, 1, 1, 2, 2, 3, 1
1Liver Disease and Liver Metabolism Lab, CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 2Proteomics Platforms, CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, 3Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS, UbiCARE

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Hier presenteren we een protocol om te verrijken, isoleren, identificeren en karakteriseren van eiwitten die zijn gewijzigd door SUMO in vivo zowel van menselijke hepatoma cellen en lever tumoren verkregen uit muizen modellen van hepatocellulaire carcinoom met behulp van SUMO-bindende entiteiten (SUBEs).

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Biochemie afgifte 153 SUMO SUBEs leverkanker hepatocellulair carcinoom hepatoma massa-spectrometrie
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