Journal
/
Medicine
/
Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm

Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm

DOI:
10.3791/60105-v

•

00:08 min

•

August 24, 2019

•

, , , , , , , ,
1Department of Surgery,University of Virginia School of Medicine, 2Department of Surgery,Ochsner Medical Center, 3Department of Surgery,University of Florida, 4The Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine

Chapters

  • 00:04Title
  • 00:33Mouse Position Preparation
  • 01:09Thorax Entry
  • 02:24Aortic Exposure
  • 03:04Elastase Exposure
  • 03:48Chest Closure and Recovery
  • 04:28Thoracic Aortic Aneurysm (TAA) Harvest
  • 06:58Results: Representative Effects of TAA Formation
  • 07:54Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a surgical protocol to consistently induce robust descending thoracic aortic aneurysms in mice. The procedure involves left thoracotomy, thoracic aorta exposure, and placement of a sponge soaked in porcine pancreatic elastase on the aortic wall.

Tags

Murine Surgical ModelTopical ElastaseDescending Thoracic Aortic AneurysmC57 Black Six MouseThoracotomyAortic DissectionPorcine Pancreatic Elastase

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A New Murine Model of Endovascular Aortic Aneurysm Repair

Reproducable Paraplegia by Thoracic Aortic Occlusion in a Murine Model of Spinal Cord Ischemia-reperfusion

A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery

Murine Left Anterior Descending (LAD) Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Standardized Technique of Aortic Valve Re-implantation for Valve-sparing Aortic Root Replacement

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of β-aminopropionitrile-induced Aortic Aneurysm and Dissection

Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique

Read Article