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JoVE Journal Cancer Research
Oprichting en karakterisering van de neuro-endocriene tumor Spheroids van de kleine darm
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Oprichting en karakterisering van de neuro-endocriene tumor Spheroids van de kleine darm

Article DOI: 10.3791/60303-v • 09:43 min • October 14th, 2019
October 14th, 2019 •
1, 1, 2, 3, 3, 1
1Department of Surgery, University of Iowa Carver College of Medicine, 2High Throughput Screening Facility, University of Iowa Carver College of Medicine, 3Department of Pathology, University of Iowa Carver College of Medicine

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Neuro-endocriene tumoren (netten) zijn afkomstig van neuro-endocriene cellen van de neurale Crest. Ze zijn traag groeiend en uitdagend voor cultuur. We presenteren een alternatieve strategie om netten van de dunne darm te kweken door ze als spheroids te kweken. Deze sferoïden hebben kleine darmen net markers en kunnen worden gebruikt voor het testen van de drug.

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Kankeronderzoek probleem 152 neuro-endocriene tumoren van de kleine darm spheroids synaptophysin chromogranine A SSTR2 immunofluorescentie immunohistochemie extracellulaire matrix rapamycine
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