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Etablering och karakterisering av små tarm neuroendokrina tumör spheroids
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Etablering och karakterisering av små tarm neuroendokrina tumör spheroids

Article DOI: 10.3791/60303-v • 09:43 min • October 14th, 2019
October 14th, 2019 •
1, 1, 2, 3, 3, 1
1Department of Surgery, University of Iowa Carver College of Medicine, 2High Throughput Screening Facility, University of Iowa Carver College of Medicine, 3Department of Pathology, University of Iowa Carver College of Medicine

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Neuroendokrina tumörer (NETs) härstammar från neuroendokrina celler i neurala Crest. De är långsamt växande och utmanande för kulturen. Vi presenterar en alternativ strategi för att odla nät från tunntarm genom odling av dem som spheroids. Dessa spheroids har små tarm nät markörer och kan användas för drogtester.

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Cancer forskning små tarm neuroendokrina tumörer spheroids synaptophysin Chromogranin A SSTR2 immunofluorescens immunohistokemi extracellulära matrix rapamycin
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