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JoVE Journal Neuroscience
Isolering af region-specifikke mikroglia fra en voksen mus hjerne halvkugle for dyb single-celle RNA Sequencing
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Isolering af region-specifikke mikroglia fra en voksen mus hjerne halvkugle for dyb single-celle RNA Sequencing

Article DOI: 10.3791/60347-v • 09:49 min • December 3rd, 2019
December 3rd, 2019 •
1, 1
1Department of Neurobiology, Stanford University School of Medicine

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Vi leverer en protokol til isolering af microglia fra forskellige dissekted regioner af en voksen mus hjerne halvkugle, efterfulgt af semi-automatiseret bibliotek forberedelse til dyb single-celle RNA sekvensering af fuld længde transkriptomer. Denne metode vil bidrage til at belyse funktionelle heterogenitet mikroglia i sundhed og sygdom.

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Neurovidenskab microglia isolation hjernen region fluorescens-aktiveret celle sortering single-celle RNA sekventering neuroimmunologi heterogenitet
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