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Isolation and Characterization of Patient-derived Pancreatic Ductal Adenocarcinoma Organoid Models
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Chapters
- 00:04Title
- 00:46Passaging of PDAC Organoids
- 04:59Characterization of PDAC Organoids
- 06:36Results: Isolation and Characterization of Patient-Derived Pancreatic Ductal Adenocarcinoma Organoid Models
- 07:55Conclusion
Patient-derived organoid cultures of pancreatic ductal adenocarcinoma are a rapidly established 3-dimensional model that represent epithelial tumor cell compartments with high fidelity, enabling translational research into this lethal malignancy. Here, we provide detailed methods to establish and propagate organoids as well as to perform relevant biological assays using these models.
