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Journal / Developmental Biology / Real-time bioluminescens billeddannelse af notch…
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Developmental Biology

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JoVE Journal Developmental Biology
Real-time bioluminescens billeddannelse af notch signalering dynamik under murine Neurogenesis
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Real-time bioluminescens billeddannelse af notch signalering dynamik under murine Neurogenesis

Article DOI: 10.3791/60455-v • 10:25 min • December 12th, 2019
December 12th, 2019 •
1, 1,2,3,4
1Institute for Frontier Life and Medical Sciences, Kyoto University, 2Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, 3Kyoto University Graduate School of Medicine, 4Kyoto University Graduate School of Biostudies

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Neurale Stem/stamceller udviser forskellige udtryks dynamik af notch signalerings komponenter, der fører til forskellige udfald af cellulære hændelser. Et sådant dynamisk udtryk kan afsløres ved realtidsovervågning, ikke ved statisk analyse, ved hjælp af et meget følsomt bioluminescens billedbehandlingssystem, der muliggør visualisering af hurtige ændringer i genudtryk.

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Udviklingsmæssige biologi real-time Imaging bioluminescens luciferase oscillation notch signalering Neurogenesis
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